Novel Mediator Proteins of the Small Mediator Complex inDrosophila SL2 Cells

The Mediator complex

What is it? The Mediator complex is an enormous, multi-subunit protein complex that regulates gene expression in eukaryotic cells. It acts as a molecular circuit board that integrates physiological and developmental signals and transmits them to RNA polymerase II (Pol II), the enzyme charged with transcription of all protein-encoding genes. Although initially discovered as a cofactor required f...

Molecular architecture of the yeast Mediator complex

The 21-subunit Mediator complex transduces regulatory information from enhancers to promoters, and performs an essential role in the initiation of transcription in all eukaryotes. Structural information on two-thirds of the complex has been limited to coarse subunit mapping onto 2-D images from electron micrographs. We have performed chemical cross-linking and mass spectrometry, and combined th...

Regulation of metabolism by the Mediator complex

The Mediator complex was originally discovered in yeast, but it is conserved in all eukaryotes. Its best-known function is to regulate RNA polymerase II-dependent gene transcription. Although the mechanisms by which the Mediator complex regulates transcription are often complicated by the context-dependent regulation, this transcription cofactor complex plays a pivotal role in numerous biologic...

The Mediator Complex and Lipid Metabolism.

The precise control of gene expression is essential for all biological processes. In addition to DNA-binding transcription factors, numerous transcription cofactors contribute another layer of regulation of gene transcription in eukaryotic cells. One of such transcription cofactors is the highly conserved Mediator complex, which has multiple subunits and is involved in various biological proces...

A novel caloric restriction mediator